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KMID : 0613820070170060805
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Journal of Life Science
2007 Volume.17 No. 6 p.805 ~ p.810
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Genetic diversity and Phenetics of Hypericum erectum Populations Using inter-simple sequence repeat Markers
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Huh Hong-Wook
Huh Man-Kyu
Kang Dong-Ho
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Abstract
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Inter simple sequence repeat (ISSR) markers were performed in order to analyse the phylogenetic relationships of eight Hypericum erectum populations in Korea. The six primers were produced 37 reproducible ISSR bands. Analysis of ISSR from individual plants of Korean H. erectum resulted in 22 polymorphic bands with 59.5%. Across populations, the mean number of alleles per locus was 1.348 and Shannon’s information index was 0.203. Population Mt. Gyeryong had the highest expected genetic diversity (0.175) among all populations. When species were grouped by eight populations, within group diversity was 0.140 (HS), while among group diversity was 0.472 (GST) on a per locus basis. The estimated gene flow (Nm) for H. erectum was very low (0.561). It is suggested that reproductive isolation by the isolation of geographical distance among H. erectum populations and genetic drift may have played roles in shaping the population structure of this species. In phenetic tree, all populations were well separated from each other. Thus, ISSR markers are very effective in classifying natural population levels of genus Hypericum in Korea.
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KEYWORD
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Hypericum erectum, ISSR markers, phylogenetic relationships
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